Heat Shock Proteins



Heat Shock Proteins (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. The images at the left and at the right correspond to one representative Hsp, i.e. the crystal structure of Hsp60 from Methanococcus maripaludis  (3los).

3D Structures of Heat Shock Proteins
Update June 2011

Small Hsp
1shs – Hsp – Methanococcus jannaschii

1gme – Hsp 17kD – wheat

2bol – Hsp – Taenia saginata

2h50, 2h53 – yHsp26 – yeast

2wj5 – rHsp20 crystallin domain – rat

2wj7, 3l1g – hHspB crystallin domain – human

3bbu – EcHsp15 - Escherichia coli – Cryo-EM

1ons – EcHsp31

3gla, 3gt6, 3guf – Hsp – Xanthomonas axonopodis

Hsp33
1hw7, 3m7m – EcHsp33 N-terminal

1i7f – EcHsp33 chaperone domain (mutant)

1xjh - EcHsp33 C-terminal - NMR

1vq0 – Hsp33 – Thermotoga maritime

1vzy – Hsp33 – Bacillus subtilis

HslV, HslU, HslJ
1e94, 1yyf – EcHslV+HslU

2kts – EcHslJ - NMR

1g41 – HiHslU – Haemophilus influenza

1im2 – HiHslU NBD

Hsp40
1c3g – yHsp40 C-terminal peptide binding domain

2qld – hHsp40

Hsp60 - the large subunit of the chaperonin molecule GroEL
3los – MmHsp60 - GroEL – Methanococcus maripaludis – closed state

3iyf - MmHsp60 - GroEL – open state

3e76 – EcHsp60 – GroEL+Tl ion – Escherichia coli

2nwc, 1oel, 1grl, 1la1 - EcHsp60 – GroEL

2eu1 - EcHsp60 (mutant) – GroEL

2cgt - EcHsp60 – GroEL+ADP+capsid assembly protein Gp31

2c7c, 2c7d - EcHsp60 – GroEL+GroES – cryo EM

1we3, 1wf4 - TtHsp60 – GroEL+GroES – Thermus thermophilus

1gr5 - EcHsp60 – GroEL – cryo EM

1gru - EcHsp60 – GroEL+ATP+GroES+ADP – cryo EM

1dkd - EcHsp60 – GroEL apical (top) domain (146 amino acids)+12-mer peptide

1dk7, 1jon - EcHsp60 – GroEL apical domain

1srv - TtHsp60 – GroEL apical domain

1ass, 1asx - Hsp60 – GroEL apical domain – Thermoplasma acidophilum

1kid - EcHsp60 (mutant) – GroEL apical domain

Hsp70 – 3 domains: N-terminus ATPase hydrolyzes ATP to ADP (NBD); hydrophobic amino acid binding domain (sub): C-terminus lid domain (lid).
3a8y – hHsp70 NBD+BAG domain regulator protein – human

3fzf - hHsp70 NBD+BAG domain regulator protein+ATP

3fzh, 3fzm - hHsp70 NBD+BAG domain regulator protein+ adenosine derivative

3fzk, 3fzl - hHsp70 NBD+BAG domain regulator protein+ purine derivative

3cqx - Hsp70 NBD+BAG domain regulator protein – mouse

3lof – hHsp70 lid

2p32 - CeHsp70 lid

3l6q - CpHsp70 NBD – Cryptosporidium parvum

3kvg - CpHsp70 NBD+AMPPNP

3l4i - CpHsp70 NBD+ADP+P

3jxu, 3i33, 3gdq, 3fe1 - hHsp70 NBD+ADP+P

3iuc - hHsp70 NBD+ADP

2e88, 1s3x, 1hjo - hHsp70 NBD

2e8a - hHsp70 NBD+AMPPNP

2qw9 – cHsp70 NBD – cow

2bup, 1bup, 1qqm, 1qqn - cHsp70 NBD (mutant)

2qwl, 2qwm - cHsp70 NBD+ADP

1qqo – cHsp70 (mutant)

2qwn, 2qwo, 2qwp, 2qwq, 2qwr - cHsp70 NBD (mutant)+ auxilin protein

2kho – EcHsp70 – Escherichia coli – NMR

1q5l - EcHsp70 sub domain+peptide - NMR

3gl1 - ScHsp70 NBD – Saccharomyces cerevisiae

3dpo – EcHsp70 sub+12-mer peptide

3dpp, 3dpq – EcHsp70 sub+20-mer peptide

3dob, 3dqg, 2op6 - CeHsp70 sub – Caenoharbditis elegans

2d2e, 3d2f – ScHsp70 NBD+SSE1(Hsp110)

2v7z – rHsp70 NBD+sub – rat

7hsc, 1ckr - rHsp70 sub - NMR

3lca – yHsp70 Ssa1 C-terminal+yTOM71

2v7y – Hsp70 NBD+sub – Geobacillus kaustophilus

Hsp90 – 4 domains: N-terminus (NTD); linker; middle (MD); C-terminus (CT)
3k97, 3hek, 3mnq – hHsp90 NTD+pyrrolidin derivative – human

3k98, 3k99, 3qtf - hHsp90 NTD+indole derivative

3r91 - hHsp90 NTD (mutant) +indole derivative

2wi1, 2wi2, 2wi3, 2wi4, 2wi5, 2wi6, 2wi7 - hHsp90 NTD+2-amino thienopyrimidine

3hhu - hHsp90 NTD+ carbamic acid derivative

3ft8, 3ft5 - hHsp90 NTD+quinazoline derivative

2k5b - hHsp90 NTD+Cdc37 – NMR - docking

3eko, 3ekr - hHsp90 NTD+dihydroxyphenyl amide

2jjc - hHsp90 NTD+pyrimidine-2-amine

3bm9, 3bmy - hHsp90 NTD+benzisoxazole

2of6 - hHsp90 NTD+triazine derivative

2qfo, 3nmq, 3r4m, 3r4n, 3r4o, 3r4p- hHsp90 NTD+pyrimidine derivative

1uy6, 1uy7, 1uy8, 1uy9, 1uyc, 1uyd, 1uye, 1uyf, 1uyg, 1uyh, 1uyi, 1uyk, 1uyl - hHsp90 NTD+purine derivative

2qg0, 2bz5 - hHsp90 NTD+sulfonamide derivative

2qg2, 2cvi, 2cvj, 2uwd - hHsp90 NTD+oxazol derivative

3d0b - hHsp90 NTD+benzamide derivative

3inw, 3inx - hHsp90 NTD+pochoxime derivative

2fwy, 2fwz, 2h55, 1uym - hHsp90 NTD+PU inhibitor

2ccs, 2cct, 2ccu, 2cdd, 2byh, 2byi, 2bsm, 2bt0, 1yc3, 1yc1, 1yc4 - hHsp90 NTD+pyrazol derivative

1byq - hHsp90 NTD+ATP-Mg

2qf6 - hHsp90 + antigen

2xhr, 2xht, 2xhx, 2xjg, 2xjj, 2xjx, 2xk2, 2xdx, 2xdu, 2xds, 2xdl, 2xdk, 2xab, 2vcj, 2vci, 3hyy, 3hyz, 3hz1, 3hz5, 3mnr - hHsp90 NTD+ antigen

3pry – hHsp90 MD

3q6m, 3q6n - hHsp90 MD + CT

2brc, 2bre, 1am1 - yHsp90 NTD+pyrazol derivative

1amw - yHsp90 NTD+ADP

1ah6, 1ah8 - yHsp90 NTD

1osf, 1yer, 1yes, 1yet - hHsp90 NTD+geldamycin

1bgq – yHsp90+radicicol

1a4h, 2weq – yHsp90+ geldamycin

3ied - PfHsp90 NTD+AMPPN – Plasmodium falciparum

3k60 - PfHsp90 NTD+ADP

2jki - Hsp90 NTD+SGT1 domain protein – barley

1sf8 – EcHsp90 C-terminal

1y4s, 1y4u, 2iop, 2ior - EcHsp90+ADP

2gq0 - EcHsp90 MD

2ioq - EcHsp90

2h8m, 2hch – dHsp90 NTD – dog

2o1w - dHsp90 NTD+MD

2hg1 - dHsp90 NTD+ligand

2o1t – dHsp90 MD+CT

2o1u – dHsp90+AMPPNP

2o1v - dHsp90+ADP

2xcm – Hsp90 NTD+CGT1+RAR1 – Hordum vulgare

3h80 – LmHsp90 NTD – Leishmania major

3q5j, 3q5k, 3q5l - LmHsp90 NTD + inhibitor

3hjc - LmHsp90 CT

3o6o, 3omu, 3opd – TbHsp90 N-terminal+inhibitor – Trypanosoma brucei

Hsp82 is the yeast equivalent of Hsp90 in mammals
1ah6, 1ah8 – yHsp82 NTD1ah6, 1ah8 – yHsp82 NTD

2xd6 - yHsp82 NTD+macrolactone

2wep – yHsp82 NTD (mutant)+ADP

2akp - yHsp82 NTD (mutant)

1weq, 3c11 - yHsp82 NTD (mutant)+ geldamycin

2wer - yHsp82 NTD (mutant)+radicicol

1bgq - yHsp82 NTD +radicicol

2iws, 2iwu, 2iwx, 2cgf - yHsp82 NTD +radicicol analogues

2vw5, 2vwc - yHsp82 NTD +7-o-carbamoylpremacbecin

3c0e - yHsp82 NTD (mutant)

2fxs - yHsp82 NTD +radamide

1zw9 - yHsp82 NTD + adenine derivative

1zwh - yHsp82 NTD + radester amine

2brc, 2bre - yHsp82 NTD+pyrazol derivative

2cg9 – yHsp82+co-chaperone SBA1

2cge - yHsp82 MD+CT+co-chaperone SBA1

1hk7 - yHsp82 MD

1usu, 1usv - yHsp82 MD+co-chaperone AHA1

1us7 - yHsp82 NTD+co-chaperone cdc37

3fp2 – yHsp82 CT+TOM71

Hsp100 are named ClpA or ClpB
1r6b, 1ksf – EcClpA (mutant) - Escherichia coli

1khy – EcClpB NTD

1jbk – EcClpB NBD

1k6k - EcClpA NTD

Hsp110 in mammals is named Sse1, Sse2 in yeast
3d2e, 3d2f, 3c7n – Sse1+Hsp70 – yeast

2qxl – Sse1 - yeast